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. 2012 Oct 1;25(5):423–432. doi: 10.1089/vim.2012.0046

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Copyright 2012, Mary Ann Liebert, Inc.

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FIG. 3. — Isotype distribution and avidity of antibodies after prime-boost immunizations. Titers of IgG₁ and IgG_2a to (A) gp160B, (B) gp140C, and (C) p17/p24B, were assessed by ELISA on pooled serum (n=6) collected 2 weeks after the last immunization. Additionally, ratios of IgG₁ to IgG_2a titers were calculated for (D) gp160B, (E) gp140C, and (F) p17/p24B. Avidity of binding antibodies to (G) gp160B, (H) gp140C, and (I) p17/p24B were determined on pooled sera (n=6) collected 2 weeks after the last immunization. Duplicates of serum dilutions were incubated in an ELISA assay followed by incubation in either urea or PBS. The difference in titers with and without incubation with urea corresponds to the avidity of antibodies (D, DNA; M, MVA-CMDR; P, rgp140C).