FIG. 5.

Effect of MVA-CMDR on rgp140C-specific immune responses. BALB/c mice were immunized with rgp140C alone or mixed with the adjuvants wild-type (wt) MVA or MVA-CMDR. rgp140C and MVA-CMDR were also delivered to different injection sites and subsequent to each other at the same injection site. (A) Binding gp140C-specific antibody titers were assessed by ELISA on sera collected 2 weeks after the third and last immunization. (B) Cell-mediated immune responses were measured by IFN-γ ELISpot on splenocytes pooled group-wise (n=5). A Gag A peptide pool was used to assess Gag-specific immune responses. Env B and E peptide pools and rgp140C were used to assess Env-specific responses.