Figure 1.

The hybrid Rnq1 proteins used to observe aggregation patterns and map prion structures. The genes encoding these proteins were located on a multicopy pYes2 plasmid under control of the GAL1 promoter. Amino acid residues 1–152: presumable non-prionogenic region, residues 153–405: QN-rich prionogenic region. Dark grey box: the prion core at residues 366–405 observed earlier [11].