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. 2024 Mar 17;25(6):3397. doi: 10.3390/ijms25063397

Figure 3.

Figure 3

Replacement of the Rnq1 protein by its truncated variants or Rnq1-GFP can cause loss of the prion. Cell lysates were loaded for electrophoresis with or without boiling and Western blotted with immunostaining for Rnq1. Prion aggregates do not enter polyacrylamide gel but dissolve when boiled. (A) Lysates of the descendants of the 74-D694 [RNQ+]-H strain with the RNQ1 gene were replaced with the indicated RNQ1 truncated versions or RNQ1-GFP. Only Rnq1-392 acquired the prion form. (B) The strains from panel A were crossed to the 21G-H67 [RNQ+]-H: all Rnq1 variants co-aggregated, except for Rnq1-277. (C) The BY4741 strain is [RNQ+], but its RNQ1-GFP descendant lacks prion, the same as 74-D694 [RNQ+]-VH with RNQ1 replaced with RNQ1-GFP.