FIG. 5.

Association of HPIV3 RNP with the CSK framework in vivo. CV-1 cells grown on coverslips were infected with HPIV3 at 1 PFU/cell, and at 12 h postinfection the cells were treated with CSK buffer containing rhodamine-conjugated phalloidin. The cells were washed with the same buffer followed by PBS and fixed. The fixed CSK structures were treated with anti-HPIV3 antibody that detects the RNP-associated proteins NP and P. The coverslips were then treated with biotin-conjugated anti-goat antibody followed by fluorescein-conjugated avidin. Similar staining of mock-infected cells served as control. Mock-infected (A) or HPIV3-infected (C) CV-1 cells treated with CSK buffer containing rhodamine-phalloidin and mock-infected (B) or HPIV3-infected (D) CV-1 cells treated with anti-HPIV3 antibody followed by biotin-conjugated anti-goat immunoglobulin plus fluorescein-conjugated avidin are shown.