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Counterstaining of GFP+ with microglial markers TMEM119 and CSF1R in the optic nerve proper. All of the GFP+ cells were co-localized with the microglia marker TMEM119 and the CSF1R. Even after 7 weeks on the PLX5622 diet, some GFP+/TMEM119+ cells persisted in the optic nerve proper. Scale bars, 50 μm.
