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Genomic structure of helper virus and the pBabeNeo vector. (a) Schematic diagram of the helper virus genome showing the locations of PCR primers used in this study (not drawn precisely to scale). The subgenomic transcript bearing the env gene is also depicted. s.d., splice donor site; s.a., splice acceptor site. (b) Schematic diagram of the pBabeNeo vector (15). A subgenomic transcript containing neo, driven by the simian virus 40 (SV40) early promoter, would also be obtained.
