TABLE 2.
Identity of packaging cell line RT PCR products
| Cell line | RT PCR for: | Product size (bp) | Database sequencea | % Similarity to database |
|---|---|---|---|---|
| FLYA4L3 | Type B or D virus | 295 | HERV-K10 | 90 |
| Type B or D virusb | 298 | HERV-K10 | 72 | |
| Type B or D virusb | 297 | HERV-K10 | 75 | |
| Type C virusc | 263 | HERV | 97 | |
| RTVL-H | 411 | RTVL-H2 | 94 | |
| Pan-retrovirus | 135 | Human RT | 66 | |
| AM12Lac25 | VL30 | 484 | VL30 | 99 |
| Type B or D virus | 298 | Murine IAP | 95 |
a
GenBank database accession numbers: HERV-K10, gb_pr:m14123; HERV, gb_pr:m74509; RTVL-H2, gb_pr:m18048; human RT, gb_pr:m25767; VL30, gb_ro:m21123; and murine IAP, gb_ro:m17551.
b
Sequences correspond to the 70A and 70B HERV-K10 families (21).
c
RNA transcripts from the CeB plasmid were also detected at approximately the same frequency as HERV transcripts.