TABLE 3.
Presence and quantification of retroviral sequences in gradient-purified virus particlesa
| Sequence | Sensitivity (fg)b | AM12Lac25 cell line
|
FLYA4L3 cell line
|
||
|---|---|---|---|---|---|
| Peak RNA level (fg) | LacZ/ERV ratio | Peak RNA level (fg) | LacZ/ERV ratio | ||
| LacZ | 527 | 6.3 × 103 | NA | 171 × 103 | NA |
| VL30 | 212 | 848 | 7.2:1 | NA | NA |
| Type B or D virus (murine) | 61 | <61 | >62:1 | NA | NA |
| Pan-retrovirus | 1.59 | <1.59 | >1,100:1 | <1.59 | >29,000:1 |
| Type C virus | 4.76 | <4.76 | >700:1 | 4.76 | 19,000:1 |
| RTVL-H | 160 | NA | NA | <160 | >880:1 |
| HERV-K10 family | |||||
| 90 | 175 | NA | NA | <175 | >580:1 |
| 70A | 87 | NA | NA | <87 | >1,200:1 |
| 70B | 160 | NA | NA | <160 | >650:1 |
a
NA, not applicable.
b
Minimum input of standard RNA which resulted in a positive RT PCR signal.