Table 2.

Publications reporting transition of secondary to antral follicles in humans and bovines (step 2).

Publication	Source	Culture Period	Culture Medium	Culture System	Biomaterial	Isolation Method
Roy et al., 1993 [104]	Human	5 days	D-MEM	2D	Agar	Enzymatic
Abir et al., 1997 [23]	Human	28 days	αMEM	2D	Extracellular matrix	Mechanical
Xu et al., 2009 [105]	Human	30 days	αMEM	3D	Alginate	Enzymatic + mechanical
Xia et al., 2015 [106]	Human	8 days	αMEM	3D	Alginate	Enzymatic + mechanical
Xiao et al., 2015 [107]	Human	40 days	αMEM	3D	Alginate	Mechanical
Yin et al., 2016 [56]	Human	30 days	αMEM	3D	Alginate	Enzymatic
Telfer et al., 2008 [21]	Human	10 days	McCoy’s 5a	V-shaped microwell	No	Mechanical
McLaughlin et al., 2014 [34]	Human	6 days	McCoy’s 5a	V-shaped microwell	No	Mechanical
McLaughlin et al., 2018 [10]	Human	23 days	McCoy’s 5a	V-shaped microwell	No	Mechanical
Xu et al., 2021 [108]	Human	42 days	αMEM	N/A	No	Mechanical
Subiran Adrados et al., 2023 [39]	Human	8 days	McCoy’s 5a	3D	Alginate	Enzymatic + mechanical
Thomas et al., 2007 [109]	Bovine	6 days	McCoy’s 5a	V-shaped microwell	No	Mechanical
McLaughlin and Telfer, 2010 [110]	Bovine	15 days	McCoy’s 5a	V-shaped microwell	No	Mechanical
Rossetto et al., 2013 [52]	Bovine	16 days	α-MEM, McCoy’s 5a and TCM-199	V-shaped microwell	No	Mechanical
Paulino et al., 2018 [53]	Bovine	18 days	TCM-199	Droplets culture	No	Mechanical

N/A: not applicable.