TABLE 2.

Effects of depletion of CD4 and CD8 lymphocytes on immunity to vaginal HSV-2 infection

Micea	Depleted	Mean % of cells ± SEM in bloodb				No. of infected micec	Mean % of vaginal epithelium infected ± SEM	Shed virus proteind	IgG anti-HSV-2e	Illness score
		Day 1		Day 14
		CD4	CD8	CD4	CD8
Nonimmune	No					9/9	2.8 ± 2.1	4,900	1.0	3.0
Immune	No	26 ± 2.5	8.1 ± 1.2	32 ± 1.5	11 ± 1.9	3/19	0.06 ± 0.4	1.2	570	0.0
	Yes	0	0.05 ± 0.05	0	1.9 ± 0.87	18/20f	0.87 ± 0.33g	62h	650	0.0

^aNine nonimmune, 19 immune, and 20 immune depleted mice were used. In each group, half of the mice were used for epithelial infection and the other half were used to measure shed virus protein titers. 

^bMeasured by flow cytometry 1 or 14 days after challenge inoculation. 

^cDetected by staining of vaginal epithelium or virus protein in vaginal lumen. 

^dGeometric mean shed virus protein titer in vaginal secretions 3 days after virus challenge. 

^eGeometric mean specific viral IgG titer in vaginal secretions 1 day before virus challenge. 

^fSignificantly larger than values in nondepleted immune mice by chi-square test (P < 0.001). 

^gSignificantly larger than values in nondepleted immune mice by one-tailed Student’s t test (P < 0.0005). 

^hSignificantly larger than values in nondepleted immune mice by Wilcoxon’s signed rank test (P < 0.0001).