TABLE 4.

Effects of depletion of CD8 lymphocytes on immunity to vaginal HSV-2 infection

Micea	Depleted	Mean % of cells ± SEM in bloodb		No. of infected micec	Mean % of vaginal epithelium infected ± SEM	Shed virus proteind	IgG anti-HSV-2e	Illness score
		CD4	CD8
Nonimmune	No			10/10	6.3 ± 2.1	4,200	1.0	3.0
Immune	No	41 ± 3.4	6.5 ± 0.6	2/19	0.08 ± 0.1	1.0	560	0.0
	Yes	50 ± 2.0	0	11/19f	1.3 ± 0.5g	6.5h	730	0.0

^aTen nonimmune, 19 immune, and 19 immune depleted mice were used. In each group, half of the mice were used for epithelial infection and the other half were used to measure shed virus protein titers. 

^bMeasured by flow cytometry 1 day after challenge inoculation. 

^cDetected by staining of vaginal epithelium or virus protein in vaginal lumen. 

^dGeometric mean shed virus protein titers in vaginal secretions 3 days after virus challenge. 

^eGeometric mean specific viral IgG titers in vaginal secretions 1 day before virus challenge. 

^fSignificantly larger than values in nondepleted immune mice by chi-square test (P < 0.002). 

^gSignificantly larger than values in nondepleted immune mice by one-tailed Student’s t test (P < 0.0001). 

^hSignificantly larger than values in nondepleted immune mice by Wilcoxon’s signed rank test (P < 0.03).