TABLE 3.
Determination of total IgA and IgG and anti-RRV antibodies in mouse sera and stools by an ELISA
| Backpack tumor | Isotype | Antigena | IgA concn (μg/ml) in:
|
IgG concn (μg/ml) in:
|
Anti-RRV antibody concnb in:
|
|||
|---|---|---|---|---|---|---|---|---|
| Serum | Stool | Serum | Stool | Serum | Stool | |||
| 1E4 | IgA | VP4 (ND) | 640 | 1.4 | 200 | —c | 16,000 | 80 |
| 2A10 | IgA | VP4 (133) | 820 | 230 | — | 32,000 | 80 | |
| 2B12 | IgA | VP4 (148) | 410 | 3.6 | 125 | — | 8,000 | 40 |
| 4B6 | IgA | VP4 (190) | 950 | 1.1 | 180 | — | 32,000 | 40 |
| 1D4 | IgA | VP6 (ND) | 710 | 0.9 | 140 | — | 64,000 | 160 |
| 2C5 | IgA | VP6 (ND) | 1,050 | 0.7 | 190 | — | 64,000 | 160 |
| 2F8 | IgA | VP6 (ND) | 460 | 1.6 | 160 | — | 32,000 | 160 |
| 1A9 | IgG | VP4 (100) | 3.2 | 1,540 | — | 128,000 | <10 | |
| 7A12 | IgG | VP4 (188) | 0.8 | 0.6 | 1,230 | — | 64,000 | <10 |
| 3C10 | IgA | Sabin 1 poliovirus | 510 | 1.2 | 190 | — | <100 | <10 |
| None | 11.2 | 1.2 | 210 | — | <100 | <10 | ||
a
See Table 1, footnote a.
b
Reciprocal of the last positive dilution in the ELISA.
c
—, The concentration was less than 0.1 μg/ml of stools.