Table 1.
Main phase transition thermodynamic parameters (Tm, ΔT1/2 and ΔH) of DMPC and DPPC LUVs, for increasing amounts of DMSO, added to already formed liposomes a.
XDMSO | % DMSO (v/v) | Tm /°C | ΔT1/2/°C | ΔH kJ·mol−1 |
Tm/°C | ΔT1/2/°C | ΔH kJ·mol−1 |
---|---|---|---|---|---|---|---|
DMPC | DPPC | ||||||
0 | 0 | 24.5 | 0.8 | 18 | 41.3 | 1.6 | 33 |
0.0018 | 0.7 | 24.5 | 0.8 | 19 | 41.4 | 1.6 | 33 |
0.0026 | 1.0 | 24.5 | 0.8 | 19 | 41.3 | 1.7 | 32 |
0.0051 | 2.0 | 24.6 | 0.8 | 19 | 41.3 | 1.7 | 32 |
0.078 | 3.0 | 24.6 | 0.8 | 19 | 41.4 | 1.7 | 31 |
0.010 | 4.0 | 24.7 | 0.9 | 18 | 41.4 | 1.8 | 31 |
0.013 | 5.0 | 24.7 | 0.8 | 18 | 41.5 | 1.8 | 32 |
0.019 | 7.0 | 24.7 | 0.9 | 17 | 41.5 | 1.8 | 31 |
0.027 | 10 | 24.8 | 1.0 | 17 | 41.6 | 1.9 | 32 |
0.060 | 20 | 25.2 | 1.7 | 17 | 41.6 | 2.6 | 30 |
0.098 | 30 | 26.2 | 3.5 | 17 | 42.4 | 2.9 | 30 |
0.14 | 40 | 27.9 | 5.2 | 17 | 42.8 | 3.2 | 27 |
a The estimated uncertainty is ±0.2 °C for the transition temperature, ±0.2 °C for the half width at half height, and ±1.5 kJ·mol−1 for ΔH.