Table 4.
Thermodynamic parameters for the main phase transition of DMPC LUVs (Tm, ΔT1/2 and ΔH) using two different SDS concentrations and DMSO contents up to 10% (v/v) a.
XDMSO | %DMSO (v/v) | Tm/°C | ΔT1/2/°C | ΔH kJ·mol−1 |
Tm/°C | ΔT1/2/°C | ΔH kJ·mol−1 |
---|---|---|---|---|---|---|---|
SDS 200 µM | SDS 500 µM | ||||||
DMPC | 24.5 | 1.0 | 18 | 24.5 | 1.0 | 18 | |
0 | 0 | 23.7 | 1.0 | 18 | 23.0 | 1.0 | 19 |
0.0078 | 3.0 | 23.7 | 1.0 | 18 | 23.0 | 1.0 | 19 |
0.013 | 5.0 | 23.7 | 1.0 | 18 | 23.1 | 1.0 | 19 |
0.019 | 7.0 | 23.8 | 1.0 | 18 | 23.2 | 1.0 | 18 |
0.027 | 10 | 23.9 | 1.0 | 18 | 23.5 | 1.0 | 17 |
a The estimated uncertainty is ±0.2 °C for the transition temperature, ±0.2 °C for the half width at half height, and ±1.5 kJ·mol−1 for ΔH.