TABLE 1.
Oligonucleotide primers used in an analysis of HPV31b gene expression in CIN-612 9E rafts
| Primer | Sequencea | Orientation | ORFb | HPV31 nta |
|---|---|---|---|---|
| P77 5′ | 5′-GCA CAT AGT CTG TGG TGC AAA CC-3′c | Sense | URR | 75–97 |
| E6 3′ | 5′-GGG TAT TTC CAA TGC CGA GC-3′ | Antisense | E6 | 173–154 |
| E7 3′ | 5′-CTG GAT CAG CCA TTG TAG TTA CAG TCT AGT AG-3′ | Antisense | E7E1 | 874–843 |
| E1 3′ | 5′-TGT CCT CTT CCT CGT GC-3′ | Antisense | E1 | 2667–2683 |
| E4-2 3′ | 5′-CGC CCG CCG CAC ACC TTC ACT GGT GCC CAA G-3′ | Antisense | E4 | 3409–3380 |
| E4 3′ | 5′-CTT CAC TGG TGC CCA AGG-3′ | Antisense | E4 | 3395–3378 |
| L2-3 3′ | 5′-GTA GAG CGT TTG GAC CGC-3′ | Antisense | L2 | 4173–4190 |
| L1-2 3′ | 5′-TAG CAC TGC CTG CGT G-3′ | Antisense | L1 | 5657–5672 |
a
Corresponding to the sequence and numbering of HPV31 (19).
b
ORF or region of HPV31.
c
Underlined bases were altered from the wild-type sequence to increase the melting temperature.