| Bacterial strains |
| BL21(DE3) cells |
New England Biolabs |
Catalog no. C2527H |
| Chemicals, peptides, and recombinant proteins |
| Glutathione Sepharose 4B |
Expedeon |
Catalog no. AGSCUST |
| GST-UBE2Q1_full length 1–422 |
MRC-PPU Reagents and Services |
DU4213 |
| GST-UBE2Q1 220–422 (end) (WT) |
MRC-PPU Reagents and Services |
DU23956 |
| GST-3C-UBE2Q1 D220-G422 C351S |
MRC-PPU Reagents and Services |
DU61423 |
| GST-3C-UBE2Q1 Y343A D220-G422 |
MRC-PPU Reagents and Services |
DU73951 |
| GST-3C-UBE2Q1 Y343F D220-G422 |
MRC-PPU Reagents and Services |
DU77761 |
| GST-3C-UBE2Q1 L345A D220-G422 |
MRC-PPU Reagents and Services |
DU73272 |
| GST-3C-UBE2Q1 L405A D220-G422 |
MRC-PPU Reagents and Services |
DU73273 |
| GST-3C-UBE2Q1 I408A D220-G422 |
MRC-PPU Reagents and Services |
DU73274 |
| GST-3C-UBE2Q1 H409A D220-G422 |
MRC-PPU Reagents and Services |
DU73264 |
| GST-3C-UBE2Q1 H409N D220-G422 |
MRC-PPU Reagents and Services |
DU73955 |
| GST-3C-UBE2Q1 N412A D220-G422 |
MRC-PPU Reagents and Services |
DU73263 |
| GST-3C-UBE2Q1 W414A D220-G422 |
MRC-PPU Reagents and Services |
DU73277 |
| GST-3C-UBE2Q1 W414F D220-G422 |
MRC-PPU Reagents and Services |
DU73954 |
| GST-3C-UBE2Q1 W414Q D220-G422 |
MRC-PPU Reagents and Services |
DU73975 |
| GST-3C-UBE2Q1 Y415A D220-G422 |
MRC-PPU Reagents and Services |
DU73276 |
| GST-3C-UBE2Q1 P417G D220-G422 |
MRC-PPU Reagents and Services |
DU73945 |
| GST-3C-UBE2Q1 P417A D220-G422 |
MRC-PPU Reagents and Services |
DU73946 |
| GST-3C-UBE2Q1 P418A D220-G422 |
MRC-PPU Reagents and Services |
DU73270 |
| GST-3C-UBE2Q1 P418G D220-G422 |
MRC-PPU Reagents and Services |
DU73271 |
| GST-3C-UBE2Q1 D421A D220-G422 |
MRC-PPU Reagents and Services |
DU73275 |
| GST-3C-UBE2Q1 D421L D220-G422 |
MRC-PPU Reagents and Services |
DU73952 |
| GST-3C-UBE2Q1 Y364F D220-G422 |
MRC-PPU Reagents and Services |
DU77760 |
| GST-3C-UBE2Q1 E367A D220-G422 |
MRC-PPU Reagents and Services |
DU77636 |
| GST-3C-UBE2Q1 E367L D220-G422 |
MRC-PPU Reagents and Services |
DU77771 |
| GST-3C-UBE2Q1 M371A D220-G422 |
MRC-PPU Reagents and Services |
DU77635 |
| GST-3C-UBE2Q1 L354A D220-G422 |
MRC-PPU Reagents and Services |
DU77640 |
| GST-3C-UBE2Q1 L354G D220-G422 |
MRC-PPU Reagents and Services |
DU77637 |
| GST-3C-UBE2Q1 Y364A D220-G422 |
MRC-PPU Reagents and Services |
DU77639 |
| GST-3C-UBE2Q1 Y364F D220-G422 |
MRC-PPU Reagents and Services |
DU77760 |
| GST 3C UBE2J2 T11-T183 |
MRC-PPU Reagents and Services |
DU61848 |
| GST 3C UBE2J2 T11-T183 D99A |
MRC-PPU Reagents and Services |
DU72870 |
| GST 3C UBE2J2 T11-T183 D99N |
MRC-PPU Reagents and Services |
DU75386 |
| GST 3C UBE2J2 T11-T183 D99K |
MRC-PPU Reagents and Services |
DU75387 |
| GST 3C UBE2J2 T11-T183 F100A |
MRC-PPU Reagents and Services |
DU72894 |
| GST 3C UBE2J2 T11-T183 H101A |
MRC-PPU Reagents and Services |
DU72871 |
| GST 3C UBE2J2 T11-T183 P102A |
MRC-PPU Reagents and Services |
DU72872 |
| His-TEV-UBE1 |
MRC-PPU Reagents and Services |
DU32888 |
| Ubiquitin-SATGSHHHHHHG |
MRC-PPU Reagents and Services |
DU21990 |
| Ubiquitin (tagless) |
MRC-PPU Reagents and Services |
DU 20027 |
| Ubiquitin L8A (tagless) |
MRC-PPU Reagents and Services |
DU71833 |
| Ubiquitin I44A (tagless) |
MRC-PPU Reagents and Services |
DU75710 |
| Ubiquitin H68A (tagless) |
MRC-PPU Reagents and Services |
DU71824 |
| Ubiquitin V70A (tagless) |
MRC-PPU Reagents and Services |
DU75713 |
| pET15b 6His HOIL |
MRC-PPU Reagents and Services |
DU32155 |
| GST-TEV-HOIP 697-1072 |
MRC-PPU Reagents and Services |
DU22629 |
| 6His SUMO parkin (223S) |
MRC-PPU Reagents and Services |
DU40847 |
| 6His 3C UBCH7 |
MRC-PPU Reagents and Services |
DU12798 |
| pET156P-1-JOSD1 |
MRC-PPU Reagents and Services |
DU20956 |
| GST-3C--USP2 |
MRC-PPU Reagents and Services |
DU13025 |
| Ac-Lys (nucleophile) |
BACHEM |
Product no. 4033018 |
| Ac-Ser (nucleophile) |
Fluorochem |
Product no. 10-F505529 |
| Ac-Cys (nucleophile) |
BACHEM |
Product no. 4031426 |
| Ac-Thr (nucleophile) |
Apollo Scientific |
Catalog no. OR36345 |
| B4GALT1 and BST-2/Tetherin peptides |
Peptide 2.0 |
Custom order |
|
Software and algorithms
|
| Prism |
GraphPad, version 10.0.3 |
https://graphpad.com/scientific-software/prism/
|
| PyMOL |
Schrodinger |
https://pymol.org/2/ |
| Adobe Illustrator |
Adobe |
https://adobe.com/uk/products/illustrator.html
|
| ChimeraX |
UCSF ChimeraX |
https://cgl.ucsf.edu/chimerax/
|
| JALVIEW |
University of Dundee |
https://jalview.org/
|
