FIG. 6.

Quantitation of replication and packaging by a TCA precipitation assay. Vero cells were infected with tsProt.A and incubated in the presence of tritiated thymidine, and then extracts were prepared after 9 h at 39°C (samples 1 and 3) or after a further 3 h at 31°C (samples 2 and 4). Samples 3 and 4 received ACV 1 h before the end of the 39°C incubation. (A) Amounts of total DNA present in each sample, determined by TCA precipitation as described in Materials and Methods. Plotted values and error bars indicate the means and standard deviations from the means for cell extracts TCA precipitated in two separate experiments, each in triplicate. (B) Amounts of DNase I-resistant (packaged) DNA present, determined as described in Materials and Methods. TCA precipitations were performed in two separate experiments, each in triplicate.