| Holding stage | 95 °C | - 5 min | ||
| Denaturation | 95 °C | - 1 min |
|
|
| Annealing | 57 °C | - 45 s | 35 | |
| Extension | 72 °C | - 1 min | ||
| Final Extension | 72 °C | - 10 min |
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