FIG. 8.
In vitro processivity of HBV Pol. HBV nucleocapsids were expressed in insect cells via infection with a recombinant baculovirus carrying a full-length HBV pregenome (ECPE) in the absence (−PFA) or presence (+PFA) of 1.5 mM PFA. Purified capsids were incubated in vitro without (−EPA) or with (+EPA) deoxynucleotides as described in Materials and Methods. Aliquots of the extracted DNA were annealed to 32P-end-labeled oligomers spanning nt 1764 to 1783 and 1382 to 1401 of the HBV sequence and extended as described above. Primer extension products were analyzed on a 6% polyacrylamide sequencing gel adjacent to sequencing ladders (lanes G, A, T, and C) generated with the analogous primers and HBV plasmid DNA. The positions of the 65- and the 447-nt extension products mapping to DR1 are indicated.