FIG. 6.
Flow cytometric analysis of intracellular HIVNL4-3 expression in A3.01 T cells. The dot plots show A3.01 T cells either cotransfected with background vector as a control (mock) or cotransfected with expression vector with or without 0.25 μg of HIVNL4-3 DNA as described above. Immunofluorescence of the A3.01 total T-cell population (A), mock-transfected cells (B), cells cotransfected with HIVNL4-3 (C), and cells cotransfected with HIVNL4-3 and RafΔ22–303-Cx (D) is shown. Numbers in the dot plots indicate percentage of p24-positive cells (FSC, forward scatter; SSC, right-angle scatter). Intracytoplasmic staining of cells with anti-p24 monoclonal antibodies was performed at 64 h posttransfection as described in Materials and Methods. The figure is representative of three independent experiments.