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. Author manuscript; available in PMC: 2024 Mar 27.
Published in final edited form as: Methods Mol Biol. 2022;2429:281–306. doi: 10.1007/978-1-0716-1979-7_19

Figure 2. COSMID in silico off-target screening.

Figure 2.

COSMID was performed for R-66 SCD gRNA that targets the HBB sequence with SCD mutation [7]. (a) Bioinformatic prediction of potential off-target sites for the gRNAs was carried out using the COSMID with up to 3 mismatches or with up to 2 mismatches and an insertion or deletion allowed in the 19 PAM proximal bases. The Homo sapiens genome assembly GRCh38/hg38 genome build was used as a reference. (b) Typical COSMID output. Only part of the output is shown.