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. Author manuscript; available in PMC: 2024 Mar 27.
Published in final edited form as: Methods Mol Biol. 2022;2429:281–306. doi: 10.1007/978-1-0716-1979-7_19

Figure 3. dsODN tag knockin rate quantification at on and off-target sites in CD34+ HSPCs by Inference of CRISPR Edits.

Figure 3.

(ICE)[8]. GUIDE-seq was performed in sickle cell disease patient derived CD34+ HSPCs for R-66 SCD gRNA that targets the HBB sequence with sickle mutation [7]. Relative contribution of alleles with dsODN tag knockin in forward orientation at (a) the on-target and (b) the active off-target CRISPR cleavage site.