Skip to main content
. Author manuscript; available in PMC: 2024 Mar 27.
Published in final edited form as: Methods Mol Biol. 2022;2429:281–306. doi: 10.1007/978-1-0716-1979-7_19

Figure 4. Example of GUIDE-seq results and off-target activity quantification by NGS in CD34+ HSPCs.

Figure 4.

(a) Visualization of GUIDE-seq output. (b) The cleavage activities at the on-target site (ON) and 57 COSMID and GUIDE-seq identified off-target sites (OT) were analyzed by NGS. The dot-plot shows indel frequencies at the on-target site (HBB) and the 7 off-target sites with measurable cleavage activity [7]. GUIDE-seq read counts correlated highly with the indel frequency quantified by NGS, with on-target sites having the highest GUIDE-seq read counts.