| 25 μL reaction | Component |
| 5 μL | 5x OneTaq standard buffer |
| 0.5 μL | 10 mM dNTPs |
| 1 μL | 5 μM i5 adapter |
| 1 μL | 5 μM i7 adapter |
| 10 μL | PCR I sample |
| 0.125 μL | OneTaq polymerase |
| 7.375 μL | Nuclease-free water |
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