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. Author manuscript; available in PMC: 2024 Mar 27.
Published in final edited form as: Methods Mol Biol. 2022;2429:281–306. doi: 10.1007/978-1-0716-1979-7_19
0.5 μL Nuclease-free water
1.0 μL 5 mM dNTP mix
2.5 μL Ligation buffer, 10x
2.0 μL End-repair mix (low)
2.0 μL 10x Taq buffer, Mg2+-free
0.5 μL Taq polymerase (non-hot start)