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. Author manuscript; available in PMC: 2024 Mar 27.
Published in final edited form as: Methods Mol Biol. 2022;2429:281–306. doi: 10.1007/978-1-0716-1979-7_19
10.7 μL Nuclease-free water
0.6 μL 10 mM dNTP mix
3.0 μL 10x Taq buffer, Mg2+-free
2.4 μL 25 mM MgCl2+
0.3 μL Platinum Taq polymerase
1.0 μL 10 μM GSP1 + or −
1.5 μL 0.5 M TMAC
0.5 μL 10 μM P5_1