25 μL reaction	Component
5 μL	5x OneTaq standard buffer
0.5 μL	10 mM dNTPs
0.5 μL	10 μM Forward primer
0.5 μL	10 μM Reverse primer
2 μL	50 ng gDNA at 25 ng/μL
0.125 μL	OneTaq Polymerase
16.375 μL	Nuclease-free water