Table 2. Viral genome and physical particle titers for rAAV2 produced with Rep variants.
| Rep variant | Replicate | qPCR titer (vg/mL) | qPCR SD (vg/mL) | ELISA titer (capsids/mL) | ELISA titer SD (capsids/mL) | Viral genome:capsid titer ratio |
|---|---|---|---|---|---|---|
| WT | A | 6.18E+11 | 5.77E+10 | 7.82E+11 | 1.04E+11 | 0.79 |
| B | 3.00E+11 | 3.85E+10 | 1.77E+11 | 8.95E+10 | 1.70 | |
| Q439T | A | 7.01E+11 | 6.47E+10 | 8.14E+11 | 4.27E+10 | 0.86 |
| B | 4.55E+11 | 2.33E+10 | 3.61E+11 | 1.21E+11 | 1.26 | |
| S110R | A | 1.45E+12 | 3.24E+10 | 1.73E+12 | 1.56E+11 | 0.84 |
| B | 1.04E+12 | 6.81E+10 | 1.43E+12 | 1.31E+11 | 0.73 |