Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Biological sample (M. musculus) | Acute mouse hippocampal slices | Xue et al., 2018 | C57BL/6-Doc2atm1Ytk;C57BL/6-Syt7tm1Nan | We are unable to distribute this strain, but a DKO strain can be generated by crossing C57BL/6-Syt7tm1Nan (available from The Jackson Laboratory) and Doc2aem1smoc (available from the Shanghai Model Organisms Center) or C57BL/6JCya-Doc2aem1 (available from Cyagen) |
| Biological sample (M. musculus) | Acute mouse hippocampal slices | The Jackson Laboratory | C57BL/6-Syt7tm1Nan | |
| Biological sample (M. musculus) | Acute mouse hippocampal slices | Sakaguchi et al., 1999; Groffen et al., 2010 | C57BL/6-Doc2atm1Ytk | Mouse line provided by the Verhage lab. We are unable to distribute this strain, and it is not available from a repository, but an almost-identical allele is available from the Shanghai Model Organisms Center: Doc2aem1smoc, and from Cyagen: C57BL/6JCya-Doc2aem1. |
| Biological sample (M. musculus) | Primary mouse hippocampal neurons | The Jackson Laboratory | C57BL/6-Syt7tm1Nan | |
| Biological sample (M. musculus) | Primary mouse hippocampal neurons | Sakaguchi et al., 1999; Groffen et al., 2010 | C57BL/6-Doc2atm1Ytk | Mouse line provided by the Verhage lab. We are unable to distribute this strain, and it is not available from a repository, but an almost-identical allele is available from the Shanghai Model Organisms Center: Doc2aem1smoc, and from Cyagen: C57BL/6JCya-Doc2aem1. |
| Recombinant DNA reagent | pF(UG) CamKII sf iGluSnFR A184V |
Vevea and Chapman, 2020; RRID:Addgene#158769 |
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| Recombinant DNA reagent | pF(UG) CamKII sf iGluSnFR S72A |
Vevea et al., 2021; RRID:Addgene#179726 |
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| Chemical compound, drug | EGTA-AM | Millipore #324628 | ||
| Software, algorithm | MATLAB 2021: SynapsEM code | Watanabe et al., 2020 | ||
| Software, algorithm | Prism 9 | |||
| Software, algorithm | Fiji: SynapsEM code | Watanabe et al., 2020 | ||
| Software, algorithm | MATLAB 2021: model code | This paper | See Figure 7—source code 1 |