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. 2024 Jan 30;9(5):e175053. doi: 10.1172/jci.insight.175053

Figure 5. Son haploinsufficiency causes a reduction of the early-stage HSPC (LSK) pool size, increased myeloid lineage–biased MPPs, decreased lymphoid lineage–biased MPPs, and increased early-stage erythroid progenitors in the bone marrow of adult Son+/– mice.

Figure 5

(A) Bone marrow cellularity from 8- to 10-week-old mice, presented as mean ± SD. n = 7, *P < 0.05 by 2-tailed t test. (B) Representative flow cytometry contour plots showing the gating scheme used to identify LSK and other subpopulations within the LSK pool of adult bone marrow. (C) Frequency of bone marrow LSK subsets presented as mean ± SD, n = 9; *P < 0.05, **P < 0.01, ***P < 0.001 by 2-tailed t test. (D) A schematic depicting differentiation of myeloerythroid progenitors (Lin–cKit+ [LK] cells) and surface markers defining the lineages and stages. (E) Flow cytometry contour plots demonstrating the gating scheme to define myeloerythroid progenitor subpopulations within CD41–FcgR– LK cells, using Endoglin, CD150, CD71, and Ter119 markers. (F) Frequency of bone marrow myeloerythroid subsets within LK cells, presented as mean ± SD, n = 5; **P < 0.01 by 2-tailed t test.