FIGURE 5.

Difference in acetylcholine‐induced relaxation before and after 18α‐Glycyrrhetinic acid, in proximal and distal segments. (a) The disparity in acetylcholine‐induced relaxations between proximal segments and distal segments is evident, with statistically sub‐significant differences observed (t _(welch) = 2.09, p = 0.05, n = 14). Notably, the distal segments exhibit higher values. (b) Post the application of 10 μmol of 18GA, the difference in acetylcholine‐induced relaxations between the proximal segments and the distal segments diminishes (t _(welch) = 0.87, p = 0.4, n = 14), with more significant relaxation inhibition in distal segments. This phenomenon may be connected to a major role of gap junctions in EDHF propagation.