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. 2024 Feb 28;627(8005):873–879. doi: 10.1038/s41586-024-07112-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — a, In vitro Ni-NTA pull-down assaying nucleosome-bound His-Halo-tagged wild-type (WT) cGAS or site C mutant cGAS (K285A/R300A/K427A) complex formation with the SPSB3-ELOBC heterotrimer. b, Size-exclusion chromatography of nucleosome-bound cGAS in complex with SPSB3-ELOBC. c, Left: Coomassie blue staining SDS-PAGE and cryo-EM analysis of the complex peak (red) shown in (b). Middle: Representative micrograph of the nucleosome-cGAS-SPSB3-ELOBC complex in vitrified ice from 9,855 raw images. Scale bar, 100 nm. Right: Zoomed-in cutaways highlighting the complex fibre. Scale bar, 10 nm. d, Selected 2D class averages of nucleosome-cGAS-SPSB3-ELOBC complex particles. Scale bar, 10 nm. e, Cryo-EM data processing flow chart. f, Corrected gold-standard Fourier shell correlation (FSC) curves of the nucleosome-cGAS-SPSB3-ELOBC complex for the 3D EM reconstruction maps. g, Angular distribution of nucleosome-cGAS-SPSB3-ELOBC complex particles included in the final reconstruction. h, Final 3D reconstruction of the nucleosome-cGAS-SPSB3-ELOBC complex; cGAS in blue, SPSB3 in green, nucleosomal DNA in lilac, histones in grey. One representative of at least three independent experiments is shown (a, b, c).

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