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. 2024 Feb 28;627(8005):873–879. doi: 10.1038/s41586-024-07112-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a, IP of Flag-tagged cGAS from HEK293T cells transfected with cGAS-Flag and HA-ubiquitin and treated with indicated siRNAs. Samples were analysed by immunoblot. Vinculin was used as a loading control. b, c, Representative confocal microscopy images of HeLa cells stained for SPSB3 (b) or CUL5 (c) and DAPI (blue). Scale bars, 20 μm. d, QIBC analysis of endogenous nuclear (left) or cytosolic (right) SPSB3 levels in HeLa cells. e, IP of GFP-tagged NLS-cGAS from HEK293T cells transfected with constructs for GFP-tagged NLS-cGAS, HA-tagged ubiquitin with or without SPSB3. Samples were analysed by immunoblot. f, Size-exclusion chromatography (SEC) and SDS–PAGE of an assembled Halo-cGAS-SPSB3-ELOBC complex. g, SEC and SDS–PAGE of an assembled cGAS-SPSB3-ELOBC complex. Data are mean ± SD (d). P values were obtained by one-way ANOVA with Šídák’s multiple comparison test (d). One representative of three (a-d) or two (e-g) independent experiments is shown.

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