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. 2024 Mar 13;627(8005):854–864. doi: 10.1038/s41586-024-07171-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — a, Gene marker analysis defined MERFISH cells clustered from only the ventricles. b, MERFISH images reveal that spatial expression of genes related to specific vCMs correlate with ventricular wall depth. c, UMAP shows pseudotime of these vCMs within the left ventricular wall. d, Gene expression of specific markers for each distinct vCM is plotted along the pseudotime axis. Colored lines indicate each gene examined (see legend above plots). BEC, blood endothelial cell; EPDC, epicardial-derived cell; IVS, interventricular septum; LEC, lymphatic endothelial cell; LV, left ventricle; RV, right ventricle; vCM, ventricular cardiomyocyte; vCM-LV/RV-AV, muscular valve leaflet vCM; VEC, valve endocardial cell; vEndocardial, ventricular endocardial; vFibro, ventricular fibroblast; VIC, valve interstitial cell; VSMC, vascular smooth muscle cell; WBC, white blood cell. Scale bar, 250 µm.