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. 2024 Mar 13;627(8005):854–864. doi: 10.1038/s41586-024-07171-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — a, Heatmap shows specific marker genes, as identified by NS-Forest2 classifier, for the 75 distinct cells across the developing heart. The distribution of these cells is shown according to age and region on bar graph. b, Cardiac single cells identified using the top 3,000 variable genes (left) and the 238 MERFISH genes (right) were visualized by UMAP which show that the transcriptional differences between the cell compartments (grey dashed lines) and classes (colored in a) are preserved with a limited set of genes. aCM, atrial cardiomyocyte; BEC, blood endothelial cell; Epi, epicardial; Fibro, fibroblast; IVS, interventricular septum; LA, left atrium; LEC, lymphatic endothelial cell; LV, left ventricle; ncCM, non-chambered cardiomyocyte; p.c.w., post conception weeks; P-RBC, platelet-red blood cell; RA, right atrium; RV, right ventricle; SMC, smooth muscle cell; vCM, ventricular cardiomyocyte; WBC, white blood cell.