Fig. 1.

The schematic diagram of the proposed unidirectional gene delivery electrospun fibrous membrane. (A) Schematic representation of the synthesis of siRNA@Au DENPs polyplexes and the fabrication of the dual-layer siRNA@GP/CS@PCL electrospun fibrous membrane consisted of the gene-loaded layer and positively charged layer. (B) Double-layer smart electrospun fibrous membranes were able to respond to the upregulated MMP-2 in the microenvironment, releasing more gene drugs and enriching them in peritendinous tissues via charge repulsion. (C) Inhibits COX-2 synthesis by targeting silencing of COX-2 mRNA, thereby alleviating inflammation.