TABLE 1.
MLV and HIV Gag protein distributions in DRG neurons when expressed separately or together with their homologous envelope precursor
Recombinant SFV used for infection | Gag/Env ratio in cellsa | % of positive cells with Gag in both soma and axonb |
---|---|---|
SFV-1-gagMLV | NAc | 98 |
SFV-C-gagMLV | NA | 98 |
SFV-1-gagMLV plus SFV-1-envMLV | 1:1 | 5 |
SFV-C-gagMLV plus SFV-1-envMLV | 9:1 | 95 |
SFV-1-gagHIV | NA | 65 |
SFV-C-gagHIV | NA | 70 |
SFV-1-gagHIV plus SFV-1-envHIV | 1:1 | 5 |
SFV-C-gagHIV plus SFV-1-envHIV | 9:1 | 65 |
Determined from quantitation of radioactive proteins in SDS gels.
In each experiment, 50 to 60% of DRG neurons were transfected. Analyses were then performed with 100 to 350 of the transfected neurons. Staining was with anti-MLV Gag antiserum or with anti-HIV Gag antibodies.
NA, not applicable.