TABLE 2.

MLV and HIV Gag protein distributions in DRG neurons when coexpressed with a heterologous Env protein

Combination of recombinant SFV	Ratio of recombinant particlesa	Gag/Env ratio in cellsb	Coexpression frequency (%)c	% Coexpressing cells with Gag in soma and axon
SFV-1-gagMLV plus SFV-1-envHIV	1:15	1:4	24	100
	5:15	3:1	46	100
	10:15	1.6:1	44	100
	10:10	NDd	61	100
SFV-1-gagHIV plus SFV-1-envMLV	20:1	1:4	NAe	70
	15:5	1:12	NA	62
	10:5	1:7	NA	60

^aMultiplicity of infection determined from titration on BHK-21 cells. 

^bDetermined from quantitation of radioactive proteins in SDS gels. 

^cStainings were with anti-MLV Gag antisera and anti-HIV antibodies for SFV-1-gag^MLV plus SFV-1-env^HIV and with anti-HIV Gag antibodies for SFV-1-gag^HIV plus SFV-1-env^MLV. 

^dND, not determined. 

^eNA, not applicable.