Table 1.

Primers used for cloning HSP90 cDNA by 5′- and 3′-RACE.

Primer name	Forward primer (5′-3′)	Reverse primer (5′-3′)	Tm (°C)
Partial Hsp90	GAYGACGARCAG TACRTSTGGGAG	TGCTTCTTGGCRG CCATGTADCCCAT	62
5′-RACE outer	GCTGATGGCGAT GAATGAACACTGa	AATGGGCTTGGTC TTGTTGAG	54
5′-RACE inner	CGCGGATCCGAA CACTGCGTTTGC TGGCTTTGATGa	GCTCATCATCATT GTGTTTGGAAG	60
3′-RACE outer	TGAAACTTGGCA TCCACGAG	GCGAGCACAGAA TTAATACGACTa	53
3′-RACE inner	GAATTCAGGAGA ACCAAAAGCACA TC	CGCGGATCCGAA TTAATACGACTC ACTCACTATAGGa	60
Hsp90 qPCR	GTCAGTTTGGTGT GGGTTTC	CCTTGGGTTTGTC CTCTTC	57
Tubulin qPCR	CTGCATGCTGTAC AGAGGAGATGT	CTCCTTCAAGAGA GTCCATGCCAA	55
GFP dsRNA	TAATACGACTCAC TATAGGGAGACAC ATGAAGCAGCACG ACCT	TAATACGACTCAC TATAGGGAGAAGT TCACCTTGATGCCC TTC	72
Hsp90 dsRNA	TAATACGACTAAC TATAGGGACCATT CGACCAGACAGTGG	TAATACGACTAAC TATAGGGGCGAGG TGATCTTCCCAGTC	72

Abbreviations used: HSP90, heat shock protein 90; RACE, rapid amplification of cDNA ends.

T7 promotor is bolded.

^aCommercial primer.