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. 2024 Mar 27;15:2692. doi: 10.1038/s41467-024-47030-z

Fig. 1. Cryo-EM structure of DSR2 in complex with DSAD1.

Fig. 1

a Domain organization of the DSR2 and DSAD1 proteins. b Cryo-EM structure of the DSR2-DSAD1 binary complex. The N-terminal SIR2 domain, Lid subdomain, and C-terminal H1, H2, H3, and H4 subdomains are colored in wheat, purple, magenta, cyan, green and blue, respectively. The DSAD1 protein is colored in orange. The lid region of the SIR2 domain responsible for the inter-dimer interaction is marked in the red box. The schematic diagram in the gray box shows the binding mode of DSAD1 to DSR2 molecules. c Overall structure of a single DSR2 molecule indicates a fishhook-like architecture (gray box). The catalytic pocket within DSR2 is marked in a black circle. The same color scheme as in (a) is used. d Detailed insights into the inter-dimer interface formed by the SIR2 domains. Key residues involved in the inter-dimer interaction are shown in stick representation.