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. 2024 Mar 27;15:2687. doi: 10.1038/s41467-024-46737-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Multiple sequence alignment of the DM10 domain from the human and C. reinhardtii proteins identified by iterative HMM-to-HMM searches. Proteins are designated by their UniProt identifiers. Coloring schemes as per ClustalX parameters with modifications. Predicted regions corresponding to β-sheets and based on RIB72 DM10 domain, are shown above the alignment. b Schematic representation of protein architectures of the DM10 domain family. Phylogenetic relationships were calculated using average distances and percent identity (PID) between DM10 modules used in the alignment. c Superimposition of C. reinhardtii RIB72 DM10 domain structure with the AlphaFold2-predicted DM10 domain structure of CAPS2. d Gene co-expression analysis of CAPS2 across non-cancerous ciliated Head and Neck Squamous cell Carcinoma (HNSC), Kidney renal clear cell carcinoma (KICH), Lung Adenocarcinoma (LUAD), Lung Squamous cell Carcinoma (LUSC), and Uterine Corpus Endometrial Carcinoma (UCEC) tissues. PCC = Pearson correlation coefficient. e Gene ontology (GO) enrichment analysis of CAPS2 co-expressed genes identified in (d). Enrichment P-values were obtained using the PANTHER Classification System for the slim cellular component devised by the PANTHER software⁵⁷. f RNA-seq ratios obtained from NIH3T3 fibroblast extracts. Ratios calculated as transcripts per million (TPM) of serum starved cells (24 h) over cycling cells. Source data are provided as a Source Data file. g Immunoblot of cell extracts from hTERT-RPE1 cells grown with or without serum. Proteins were probed with indicated antibodies. The shown immunoblot is representative of two independent experiments. h Immunofluorescence microscopy micrographs of motile cilia on human bronchial epithelial cells imaged by structured illumination microscopy. Cells were probed with the indicated antibodies. The images are representative of two independent experiments. Source data are provided as a Source Data file. Scale bar: 5 μm in the top row, 1 μm in the second row.