FIG. 1.

Ethidium bromide-stained agarose gel showing synthesis of DNA across the gapped-duplex DNA substrate with recombinant, purified HIV-1 RT. Complete synthesis of DNA across the gapped-duplex DNA substrate was confirmed on a 0.8% agarose gel run at 25 V for 20 h. The synthesis is indicated by the gel mobility shift observed between the initial gapped-duplex DNA (no RT) and the products obtained after filling in by wild-type, Q151M, and the VILYM RTs. ds, double stranded.