FIG. 2.

Spectra of mutations generated by wild-type and nucleoside analog-resistant HIV-1 RTs. The wild-type lacZα sequence is represented, with the corresponding nucleotide positions indicated below the sequence. Mutations created by the wild-type HIV-1 RT are shown above the lacZα sequence, and those generated by one of the mutant RTs are shown below the sequence. Base substitutions are noted directly above or below the lacZα sequence and represent the nucleotide in the mutated template. Frameshift mutations are indicated with an upright triangle for single-base deletions and with an inverted triangle for single-base insertions. Since it cannot be determined exactly which base in a run of nucleotides has been deleted or inserted, the frameshift events are indicated in the middle of the run. (Top) Comparison of the spectra of mutations generated by the wild-type and Q151M variant HIV-1 RTs. (Bottom) Comparison of wild-type and VILYM RTs. In addition to the mutations shown here, two templates generated by the VILYM RT contained large deletions (see text).