TABLE 3.
Summary of error rates for various classes of mutations
| Mutation type | Wild-type RT
|
Q151M RT
|
VILYM RT
|
|||
|---|---|---|---|---|---|---|
| No. of errors | Error rate | No. of errors | Error rate | No. of errors | Error rate | |
| All classes | 123 | 1/22,000 | 104 | 1/25,000 | 106 | 1/44,000 |
| Frameshifts | 28 | 1/61,000 | 17 | 1/99,000 | 32 | 1/94,000 |
| At runs | 25 | 1/34,000 | 15 | 1/55,000 | 27 | 1/56,000 |
| At nonruns | 3 | 1/290,000 | 2 | 1/430,000 | 5 | 1/310,000 |
| Base substitutionsa | 95 | 1/13,000 | 87 | 1/14,000 | 72 | 1/30,000 |
| Misalignment | 60 | 1/5,800 | 48 | 1/7,100 | 37b | 1/17,000 |
| Miscoding | 65 | 1/19,000 | 53 | 1/23,000 | 53 | 1/41,000 |
a
The total number of base substitutions does not equal the sum of the numbers possibly arising from misalignment or miscoding, because some mutations could not be classified as one or the other and were put in both classes. See text for details.
b
P < 0.05 by Fisher’s exact test when compared to errors made by the wild-type enzyme.