FIG. 3.

Infection of cell lines with Edmonston laboratory and Montefiore 89 wild-type strains of measles virus. HeLa cells, Vero cells, Chinese hamster ovary cells transfected with an empty expression vector (CHO-pDRα2), Chinese hamster ovary cells expressing human CD46 (CHO-CD46), and a marmoset B-cell line (B95-8) were inoculated with either Edmonston or wild-type Montefiore 89 strains of measles virus. The cells were incubated for 72 h with either strain of virus, and infection was monitored by immunoblot analysis with a monoclonal antibody to the measles virus M protein (40 kDa). (A) HeLa, Vero, CHO-CD46, and B95-8 cells supported infection by the Edmonston strain, while CHO cells transfected with the expression vector alone (CHO-pDRα2) were not infected. (B) HeLa, Vero, CHO-pDRα2, and B95-8 cells were inoculated with the wild-type Montefiore 89 strain, and infections were allowed to proceed for 72 h. Only the B95-8 cells supported infection. Protein standards (in kilodaltons) are shown at the left of each panel.