FIG. 6.

Quantitation of Edmonston H and Montefiore H binding to mouse cells expressing human CD46, marmoset/human chimeric CD46, or CD21 or to B95-8 marmoset cells. Sf9 insect cells expressing Edmonston (A) or Montefiore 89 (B) H proteins were incubated with B95-8 marmoset cells or mouse L cells expressing CD46, chimeric CD46, or CD21 as described in the legend to Fig. 5. Loosely attached cells were washed away, and binding was measured colorimetrically with the ONPG substrate for β-galactosidase. Edmonston H bound to marmoset B95-8 cells and mouse cells expressing human CD46 and chimeric CD46. It did not bind to mouse cells expressing CD21 Montefiore H bound to marmoset B95-8 cells but did not adhere to mouse cells expressing human CD46, chimeric CD46, or CD21. Binding is expressed as a percentage relative to either Edmonston H binding or Montefiore H binding to B95-8 cells.