Figure 5.

DVB neurite outgrowth induced using genetic developmental sleep disruption is transient

(A) Schematic of experimental set-up for day 3 imaging of DVB neuron in sleep deprivation mutants, aptf-1 (orange) and npr-1(blue), and L4 vibration sleep deprived males (pink).

(B) Graph showing DVB neurite length and (C) junctions in day 3 males in controls and aptf-1 males.

(D) Day 3 DVB neurite length and (E) junctions in controls and npr-1 males.

(F) Graph of neurite length and (G) number of junctions in day 3 control males and males after application of vibration during L4 to adult molt.

(H) Representative images of day 3 DVB neurons in control, aptf-1, npr-1, and L4 vibrated males. The effect of nrx-1 and nlg-1 mutations on day 3 DVB neurite outgrowth was not tested as there was no difference between sleep deprived and control C. elegans. White asterisk marks PVT neuron (not traced, see⁶⁴).Number of individual animals is indicated by “n”, p values from two-tailed unpaired t-test shown, error bars represent SEM, scale bar size as labelled.