FIG. 4.

Chromatin condensation in primary cultures of mouse pulmonary epithelial cells infected with SeV. Primary cultures of mouse pulmonary epithelial cells were infected with either M1 (a to d) or MVC11 (e to h). At 2 or 6 days p.i., cells were fixed with ethanol and subjected to immunofluorescence staining with anti-SeV rabbit antiserum (αSeV) as the first antibody and fluorescein isothiocyanate-conjugated goat anti-rabbit IgG as the second antibody for detection of virus antigens (a, c, e, and g), followed by staining with Hoechst 33342 for detection of chromatin condensation (b, d, f, and h). The arrows in panel d show the nuclei of dividing cells infected with M1, and the arrowheads in panels f and h depict chromatin condensation in the nuclei.