FIG. 2.

Comparison of CTL targets in C57BL/6 mice infected with wild-type or E1-deleted adenovirus. Splenocytes isolated on day 7 after infection of C57BL/6 mice with wild-type adenovirus (Ad5wt) (10⁹ particles per injection) (B and D) or E1-deleted adenovirus expressing ALP (AdALP) (10¹¹ particles per injection) were analyzed for specific lysis on target cells infected with recombinant vaccinia virus expressing hexon, penton, fiber, or a rabies glycoprotein (C and D) or on target cells infected with AdALP or Ad5wt (A and B) in a 6-h ⁵¹Cr release assay. Specific lysis was assayed at five different effector/target cell ratios. These data are from one of two experiments done. The standard deviations were less than 10%. Spontaneous release ranged from 10 to 15%.